Controlled enzymatic synthesis of oligonucleotides
2024年6月18日 · Controlled enzymatic synthesis is a method for the de novo production of oligonucleotides that combines elements of solid-phase synthesis and of enzymatic polymerization of nucleotides (Fig....
An enzymatic oligonucleotide synthesizer | Nature Methods
2018年7月31日 · Reversible primer termination, enabled by polymerase–nucleotide conjugates, provides an enzymatic method for the de novo synthesis of oligonucleotides. Over the past decade...
Template-independent enzymatic synthesis of RNA oligonucleotides …
2024年7月12日 · Here, we report the development and optimization of an aqueous-based, template-independent enzymatic RNA oligonucleotide synthesis platform as an alternative to traditional chemical...
Template-Independent Enzymatic Oligonucleotide Synthesis …
In this Perspective, we first evaluate the origins of enzymatic oligonucleotide synthesis; and with the knowledge gained from prior research, we explore the method defined as template-independent enzymatic oligonucleotide synthesis (TiEOS).
An enzyme cascade enables production of therapeutic oligonucleotides …
2023年6月15日 · We report a biocatalytic approach to efficiently produce oligonucleotides in a single operation where polymerases and endonucleases work in synergy to amplify complementary sequences embedded within catalytic self-priming templates. This approach uses unprotected building blocks and aqueous conditions.
oligonucleotide synthesis. The chemistry of oligonucleotide synthesis DNA or RNA consists of deoxyribose or ribose sugar units — with their characteristic bases (A, C, G, T or U) — linked together by phosphodiester bonds. Traditional oligonucleotide synthesis uses phosphoramidite chemistry and comprises a repeating sequence of four reactions
Large-Scale de novo Oligonucleotide Synthesis for Whole …
2021年6月22日 · In this review, we first summarize the status of large-scale oligonucleotide synthesis technologies including the basic methodology and large-scale synthesis approaches, with special focus on the emerging enzymatic methods.
eRNA: Controlled Enzymatic RNA Oligonucleotide Synthesis
Their scalable, flexible, and cost-efficient synthesis method uses a set of proprietary engineered enzymes and novel nucleotide building blocks to produce accurate, high-quality RNA oligonucleotide sequences that can include both natural and non-natural bases at …
Automated high-throughput DNA synthesis and assembly
2024年3月30日 · Oligonucleotide synthesis is a multi-step, continuous reaction in which nucleotide monomers are gradually added to solid-phase supports such as controlled pore glass (CPG) or polystyrene (PS) beads. Each cycle includes four critical reactions: deprotection, coupling, capping and oxidation.
Enzymatic synthesis of RNA oligonucleotides - Nature
2024年7月12日 · Controlled enzymatic synthesis of oligonucleotides is an alternative, de novo approach that merges solid-phase chemical synthesis and polymerase-based incorporation of nucleoside...