A Supramolecular Approach to Engineering Living Cells with Enzymes …
4 天之前 · Biocatalytic transformation in nature is inherently dynamic, spontaneous, and adaptive, enabling complex chemical synthesis and metabolism. These processes often involve supramolecular recognition among cells, enzymes, and biomacromolecules, far surpassing the capabilities of isolated cells and enzymes used in industrial synthesis.
Engineering enzyme conformation within liquid-solid hybrid ...
6 天之前 · The engineering of an enzyme’s structural conformation and dynamic properties to promote its catalytic activity and stability outside cellular environments is challenging. Here, the authors ...
Journal of Agricultural and Food Chemistry
6 天之前 · According to synthesis experiments, the formation of Lac-AAs was catalyzed by endoenzymes, with an optimal pH of 5–7 and a temperature of 50–60 °C. After BLAST comparison, cytosolic nonspecific dipeptidase 2 (CNDP2) or other similar metallodipeptidases may be potential enzymes for Lac-AAs synthesis.
Enzyme Synthesis - an overview | ScienceDirect Topics
As far as we know at present, all enzymes are protein in nature, and their synthesis involves the linking together of amino acids in correct sequence. Each animal carries within its body cells, in the DNA molecules, coded information for the building up of its own specific proteins.
Engineered enzymes for the synthesis of pharmaceuticals and
2024年1月4日 · In this Review, we introduce these mutagenesis strategies and then discuss individual cases of enzyme-catalysed syntheses of chiral therapeutic drugs and other value-added products as well as the...
Enzyme engineering for biocatalysis - ScienceDirect
2024年2月15日 · Over the past 25 years, there has been an unprecedented advancement in protein engineering tools, empowering enzymologists to customise enzymes to precisely meet the demands of organic synthesis. In this discussion, we delineate some of the most crucial techniques in enzyme engineering and their significance in facilitating chemical synthesis. 1.
Enzyme Synthesis - an overview | ScienceDirect Topics
Each cell is equipped with a mechanism that regulates the synthesis of enzymes in an economical way, enabling the cell to respond adequately to environmental changes. The basic mechanism of enzyme synthesis includes transcription, translation, and posttranslational processing which is highly conserved (Rhem and Reed, 1985). However, several ...
Enzymatic strategies for asymmetric synthesis - RSC Publishing
In this article, the major strategies relying on enzymes for the key step of asymmetric synthesis are reviewed and classified in one of the following categories: (i) stereoselective reactions involving transformations of sp 2 hybridized carbon atoms, (ii) enantioselective reactions and (iii) atroposelective reactions. For each category, the ...
Enzymatic DNA synthesis enters new phase - Nature
2020年10月5日 · Today, every strand of DNA manufactured outside of a cell is produced via a technique known as phosphoramidite synthesis. This process, developed by Marvin Caruthers and colleagues at the...
Multiplex enzymatic synthesis of DNA with single-base resolution
2023年7月7日 · Enzymatic DNA synthesis (EDS) is a promising benchtop and user-friendly method of nucleic acid synthesis that, instead of solvents and phosphoramidites, uses mild aqueous conditions and enzymes.