Phenylthiohydantoin - an overview | ScienceDirect Topics
In this process, the terminal amino acid is cyclized to the corresponding phenylthiohydantoin derivative (PTH-amino acid), which can be identified by gas chromatography, reverse-phase high-pressure liquid chromatography, thinlayer chromatography, or …
Phenylthiohydantoin | C9H8N2OS | CID 700731 - PubChem
Phenylthiohydantoin | C9H8N2OS | CID 700731 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Phenylthiohydantoin - an overview | ScienceDirect Topics
Enzalutamide contains the linear-linked tricycle, phenyl-thiohydantoin-phenyl. In the study, thiohydantoin and the phenyl ring of enzalutamide were cyclized with a methylene group to form indoline thiohydantoin (32), which is a rigidified form of enzalutamide.
Edman degradation - Wikipedia
Phenyl isothiocyanate is reacted with an uncharged N-terminal amino group, under mildly alkaline conditions, to form a cyclical phenylthiocarbamoyl derivative. Then, under acidic conditions, this derivative of the terminal amino acid is cleaved as a thiazolinone derivative.
Determination of sequence and absolute configuration of ... - Nature
2022年6月18日 · We report a method for the simultaneous determination of the sequence and absolute configuration of peptide amino acids using a combination of Edman degradation and HPLC–MS/CD....
解密蛋白质序列：Edman降解法讲解N端序列分析的原理与应用
2023年8月1日 · Edman降解法是由瑞典化学家Pehr Victor Edman在1950年代发展的，用于测定蛋白质或多肽的N端氨基酸序列。 它的基本步骤如下： 1.1将蛋白质或多肽在酸性条件下与Edman试剂（phenylisothiocyanate）反应，形成一个稳定的酮基硫代苯并酮（phenylthiohydantoin，PTH）。 1.2通过加热使得酮基硫代苯并酮与蛋白质断裂，获得一个PTH-氨基酸衍生物和一个缩短了一个氨基酸的蛋白质或多肽。 1.3使用高效液相色谱（HPLC） …
Phenylthiohydantoin - (Organic Chemistry) - Vocab, Definition ...
Explain the role of phenylthiohydantoin in the Edman degradation process for peptide sequencing. Phenylthiohydantoin is a key intermediate formed during the Edman degradation, a method used to sequence peptides and proteins.
PHENYLTHIOHYDANTOIN | C9H8N2O2S - ChemSpider
ChemSpider record containing structure, synonyms, properties, vendors and database links for PHENYLTHIOHYDANTOIN
苯基硫代乙内酰脲-天冬氨酸 - 百度百科
苯基硫代乙内酰脲-天冬氨酸是化学物质，CSA号是5624-13-5。
Phenylthiohydantoins in protein analysis - PubMed
Ann N Y Acad Sci. 1960 Aug 31;88:602-10. doi: 10.1111/j.1749-6632.1960.tb20056.x.