Extensive germline genome engineering in pigs - Nature
2020年9月21日 · Pigs can be genetically modified to inactivate endogenous retroviruses and to display enhanced compatibility with the human immune system using a combination of CRISPR–Cas9 and transposon ...
Generation and characterization of genetically modified pigs
6 天之前 · In this study, we generated transgenic pigs expressing human CD47 (hCD47) and CD55 (hCD55) in α1,3-galactosyltransferase KO/β-1,4-N-acetyl-galactosaminyl transferase 2 KO/cytidine...
Genetic modifications of pigs for medicine and agriculture
The most popular method of producing genetically modified pigs to date is through genomic modification of somatic cells followed by nuclear transfer (NT), first reported by Park et al. (2001). In this process, the nuclei of somatic cells are transferred into enucleated metaphase II oocytes, and then this complex is activated by electrofusion.
Extensively edited pigs | Nature Biomedical Engineering
2021年2月12日 · By using CRISPR and transposon constructs, pigs have been genetically modified to inactivate endogenous retroviruses and to enhance the compatibility of their organs with the human immune and...
Frontiers | Genetic engineering of pigs for xenotransplantation to ...
2022年12月6日 · Genetically modified pigs have been considered as potential animal donors for human xenotransplantation and have been widely used in preclinical research. The genetic modifications aim to prevent the major species-specific barriers, which include humoral and cellular immune responses, and physiological incompatibilities such as complement and ...
Genome Editing of Pigs for Agriculture and Biomedicine
2018年9月3日 · Two ways to generate genome-edited pigs are shown: embryo injection of engineered nucleases and somatic cell nuclear transfer. In genome editing process, engineered nucleases induce a DSB at the target site. Subsequent DNA-repair by NHEJ and HDR pathways can introduce site-specific knockout and knockin, respectively, in the pig genome.
Current status of the application of gene editing in pigs - PMC
This review describes the production of genetically modified pigs using gene editors, provides an overview of approaches for the generation of genetically modified pigs using various types of gene editors, and highlights current trends, including the establishment of disease models and research on pig-to-human transplantation, as well as the ...
Improvements in pig agriculture through gene editing
2022年6月21日 · Novel genetic variation and exogenous traits from outside of the genera and species can now be introduced into pig and other livestock species by using gene editing technologies.
FDA Approves First-of-its-Kind Intentional Genomic Alteration in …
2020年12月14日 · Today, the U.S. Food and Drug Administration approved a first-of-its-kind intentional genomic alteration (IGA) in a line of domestic pigs, referred to as GalSafe pigs, which may be used for...
Genetically modified pigs as donors of cells, tissues, and organs …
2019年6月25日 · Numerous genetically (multi-)modified pig lines have been generated to prevent immune rejection of xenotransplants, to overcome physiological incompatibilities, and to reduce the risk of transmitting zoonotic pathogens (Table 1; reviewed in Kemter et al., 2018). Table 1. Selection of genetic modifications of donor pigs for xenotransplantation.
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