Fig 4. NG2-staining and pericyte counts after BRVO. (A-D)...
NG2 staining of control retina shows a strong signal of the cell body around PC nuclei (Fig 4 B inset) and reveals the thin elongated processes of the PCs that cover the capillaries (Fig 4 A...
Analysis of NG2 as a Marker for Retinal Pericytes | IOVS - ARVO …
We have examined the use of NG2 as a marker for pericytes in developing and mature retinal vasculature. Methods: Retinas from mice of different age (from postnatal day 2 to adult) as well as those treated with 75% oxygen for 5 days and then recovered in room air for 5 days were stained with anti-NG2, isolectin-IB4 and anti-GFAP.
Retinal Angiogenesis Regulates Astrocytic Differentiation in …
2017年12月14日 · NG2 expression is not restricted to astrocytes or their progenitors, but also includes pericytes 21, 22. On the other hand, PDGFR-α is specifically expressed in immature and differentiated...
αSMA (A in red) or NG2 (B in red) staining of whole
αSMA (A in red) or NG2 (B in red) staining of whole-mounted P5 retinas. Lifeact-EGFP: green. Arrows point to αSMA-positive cells (A) and NG2-positive pericytes (B). Scale bars: A: 50 µm, B:...
Staining of cross-sections and flat-mounts with antibodies against ...
NG2, CD31 and GFAP staining is localized to the retrolental cell mass, indicative of the hyaloid, in transgenic retinas (D, F, M–O) compared to wild-type retinas (C, E).
Nerve/glial antigen (NG) 2 is a crucial regulator of intercellular ...
2018年1月1日 · The analysis of the underlying mechanisms revealed an activation of the MAPK signaling pathway after NG2 silencing. We further detected an increased ICAM-1 protein level in the retinal tissue of NG2 −/− mice when compared to wild-type (NG2 +/+) controls. Finally, we could demonstrate that this effect is not specific for pericytes but also ...
Circular RNA-ZNF532 regulates diabetes-induced retinal pericyte ...
2020年6月6日 · To identify high glucose–regulated circRNAs, we incubated human retinal pericytes with 5.55 mM glucose (normal glucose, NG), 5.55 mM glucose plus 24.45 mM pyruvate (osmotic control, OS), or 30 mM glucose (high glucose, HG) for 24 hours, and then prepared RNA samples for circRNA expression profiles.
NCK-dependent pericyte migration promotes pathological
2018年8月27日 · Here we show that abnormal, α-SMA-expressing pericytes cover angiogenic sprouts and pathological neovascular tufts (NVTs) in a mouse model of oxygen-induced retinopathy. Genetic lineage tracing...
Markers for human brain pericytes and smooth muscle cells
2018年10月1日 · Confocal imaging confirmed the presence of NG2 on PDGFRβ-positive capillary pericytes and αSMA-positive vSMCs, however staining was weak and punctate with no continuous staining observed (Fig. 4 g, h). These results indicate that there is uneven distribution of these markers in different mural cell phenotypes.
Pericytes in the Retina - SpringerLink
2019年4月2日 · In 2001, NG2 proteoglycan (neural glial antigen 2, CSPG4) was first described as a PC-specific marker (Ozerdem et al. 2001), showing its impact on angiogenesis by reduced proliferation of both PCs and ECs in the retina of NG2 null mice (Ozerdem and Stallcup 2004). Until now, NG2-based mouse models have been used predominantly in the ...