Characterizing the Composition of Molecular Motors on Moving …
2014年10月30日 · A protocol is presented here, which determines the motor composition (type and relative amount of motors) of individual moving vesicles from immunofluorescence (IF) data measuring endogenously expressed motor proteins, and correlates these parameters to the live transport of the exact same vesicles in neurons 16. This method entails precise ...
Stable Kinesin and Dynein Assemblies Drive the Axonal Transport …
2011年2月18日 · We identify Kinesin-1 and cytoplasmic dynein as major PrP C vesicle motor complexes and show that their activities are tightly coupled. Regulation of normal retrograde transport by Kinesin-1 is independent of dynein-vesicle attachment and requires the vesicle association of a complete Kinesin-1 heavy and light chain holoenzyme.
Axonal transport and neurodegenerative disease: vesicle-motor …
2014年3月10日 · In this review, we focus on the assembly of the amyloid precursor protein, huntingtin, mitochondria, and the RNA-motor complexes and discuss how these may be regulated during long-distance transport in the context of neurodegenerative disease.
Colocalization of different neurotransmitter transporters on …
In this study, we visualized different VTs at the single-vesicle level, resulting in a comprehensive and quantitative colocalization map of transporters and revealing the extent and diversity of corelease in the adult rodent brain. We have also studied one example of corelease to understand the benefits and relevance for synaptic physiology.
Preferential binding of a kinesin-1 motor to GTP-tubulin–rich ...
In vitro studies revealed approximately threefold stronger binding of KIF5 motor head to GMPCPP microtubules than to GDP microtubules. Collectively, these data suggest that the abundance of GTP-tubulin in axonal microtubules may underlie selective KIF5 localization and polarized axonal vesicular transport.
Polarized transport requires AP-1-mediated recruitment of KIF13A …
2024年3月6日 · A vesicle transport event is initiated by two steps: 1) cargo sorting and vesicle budding from the donor compartment, and 2) motor-based transport to the cellular destination. Because these steps are strictly successive, they must be mechanistically linked; that is, the proteins required for proper transport must be determined during vesicle ...
Coordination of two kinesin superfamily motor proteins, KIF3A …
2022年3月1日 · MT1-MMP promotes cancer invasion by degrading barrier ECM at the leading edge, and its localization is carried out by direct vesicle transport of MT1-MMP containing vesicles along the microtubule. We identified KIF3A, KIF13A, and KIF9 as kinesins involved in MT1-MMP-containing vesicle trafficking in HT1080 cells.
Single Molecule Imaging Reveals Differences in Microtubule …
2009年10月13日 · We evaluated the ability of kinesin motors involved in vesicle transport to read microtubule heterogeneity by using single molecule imaging in live cells. We show that individual Kinesin-1 motors move preferentially on a subset of microtubules in COS cells, identified as the stable microtubules marked by post-translational modifications.
Single-Axonal Organelle Analysis Method Reveals New Protein–Motor …
2013年2月2日 · We examined the colocalization of three synaptic vesicle proteins, p38, SV2A, and VAMP2, with KIF1A, the motor implicated in the transport of most synaptic vesicle proteins.
Microtubule‐binding protein doublecortin‐like kinase 1 (DCLK1) …
Growing evidence suggests that microtubule‐associated proteins may regulate motor protein transport. Here we show that DCLK1 promotes KIF1‐mediated cargo trafficking into dendrites. Our study demonstrates that microtubule‐binding proteins can provide local signals for specific kinesin motors to drive polarized cargo transport in neurons.