Three-dimensional characterization of developing and adult ocular ...
2022年10月27日 · From a single arterial source in the optic nerve the HVS emerges and diverges into three subnetworks: the vasa hyaloidea propria (VHP) in the vitreous, the tunica vasculosa lentis (TVL) over...
Anatomy of the complete mouse eye vasculature explored by light …
2023年12月1日 · Here we describe a workflow of optical tissue clearing, antibody labeling and LSFM imaging that together with in silico dissection permit visualization and analysis of the complete mouse eye vasculature in 3D.
Assessment and characterization of hyaloid vessels in mice
2019年5月5日 · Here we describe the procedures for imaging hyaloid in live mice with optical coherence tomography (OCT) and fundus fluorescein angiography (FFA) and detailed technical protocol of isolating and flat mounting hyaloid ex vivo for quantitative analysis.
Morphology of the Murine Optic Nerve | IOVS | ARVO Journals
Studies in glaucomatous mice might help to identify the importance of the lamina cribrosa and the choroidal vascular supply for optic nerve damage in glaucoma. To study the detailed patho-mechanisms of glaucoma in humans, animal models provide powerful tools.
Compartmentalized ocular lymphatic system mediates eye–brain
2024年2月28日 · Mouse optic nerve sheaths were dissected after mice were anaesthetized and transcardially perfused with cold PBS and 4% PFA (Sigma-Aldrich) sequentially.
Progressive Optic Neuropathy in Hydrocephalic Ccdc13 Mutant Mice …
Purpose: Optic nerve head (ONH) atrophy is frequently associated with hydrocephalic conditions. Cerebrospinal fluid (CSF)-containing meninges form a subarachnoid space that terminates at the ONH, which physically impacts it. This study aims to characterize optic neuropathy in congenital hydrocephalic mice with genetic disruption of the Ccdc13 gene.
Optic Nerve Crush in Mice to Study Retinal Ganglion Cell Survival …
2020年3月3日 · Herein, we describe a step-by-step protocol to assay RGC survival and regeneration in mice following AAV2-mediated transduction and ONC injury including 1) intravitreal injection of AAV2 viral vectors, 2) optic nerve crush, 3) cholera-toxin B (CTB) labeling of regenerating axons, 4) optic nerve clearing, 5) flat mount retina immunostaining, and ...
A practical approach to optic nerve crush in the mouse
2012年7月27日 · Optic nerve crush (ONC) has advantages over other methods, such as optic nerve transections, for it is relatively mild and does not interrupt ocular blood flow. The ONC is particularly useful as a simple synchronous approach for examining ganglion cell injury in a large number of mouse strains [2].
A Revised View on Growth and Remodeling in the Retinal ... - Nature
2019年3月1日 · The initial outgrowth of the retinal vasculature towards the periphery starts around p3 from the optic nerve head, where it covers a ratio of 0.16 ± 0.01 of the total collapsed retinal area.
Utilizing mouse optic nerve crush to examine CNS remyelination
2021年9月17日 · Here, we describe procedures to promote axonal regeneration, administer optic nerve crush, and assess oligodendrocyte differentiation and maturation into myelination-competent oligodendrocytes. This protocol allows for testing the efficacy of remyelination treatments in an in vivo central nervous system (CNS).