Real-Time High Resolution 3D Imaging of the Lyme Disease
2008年6月20日 · We engineered a fluorescent strain of Borrelia burgdorferi, a Lyme disease pathogen, and used conventional and spinning disk confocal intravital microscopy to directly visualize these bacteria in real time and 3D in living mice. We found that spirochete interaction with and dissemination out of the vasculature was a multi-stage process of ...
Is There a Role for Dark Field Microscopy in the Diagnosis of Lyme ...
Dark field microscopy is a diagnostic technique to visualize the spirochetes that cause Lyme disease; however, it is not recommended for the diagnosis of Lyme disease. One of the main limitations of dark field microscopy is its low sensitivity.
Metamorphoses of Lyme disease spirochetes: phenomenon of
Using confocal and epifluorescence microscopy, the authors confirmed that during the blood-feeding Borrelia spirochetes progress through the nymphal midgut as epithelial cell-associated networks of non-motile organisms.
The unusual cell wall of the Lyme disease spirochaete - Nature
2021年11月24日 · We used atomic force microscopy (AFM) to analyse purified peptidoglycan sacculi and found that A3/chbC peptidoglycan was jagged and frayed, compared with smooth, WT, peptidoglycan sacculi (Fig....
Laboratory confirmation of Lyme disease - PMC - PubMed …
Lyme disease can be confirmed in the laboratory by isolation or detection of its causative agent, a tick-borne spirochete Borrelia burgdorferi, or by a diagnostic change in the titre of antibodies specific to the agent. B burgdorferi can be isolated and cultivated in …
Pathology Outlines - Borrelia burgdorferi / Lyme disease
2016年2月1日 · The spirochetes may induce host cells to produce quinolinic acid, which stimulates NMDA receptors and manifests as malaise in lyme encephalopathy (Neurology 1992;42:43) Note: Lyme disease spirochetes are never seen in peripheral blood
Real-time high resolution 3D imaging of the lyme disease
2008年6月20日 · Due to their slender morphology, spirochetes are difficult to visualize by standard light microscopy, impeding studies of their behavior in situ. We engineered a fluorescent infectious strain of Borrelia burgdorferi, the Lyme disease pathogen, which expressed green fluorescent protein (GFP).
Characterization and genomic analysis of the Lyme disease ... - PLOS
2024年4月1日 · Lyme disease is a tick-borne infection caused by the spirochete Borrelia (Borreliella) burgdorferi. Borrelia species have highly fragmented genomes composed of a linear chromosome and a constellation of linear and circular plasmids some of which are required throughout the enzootic cycle.
Intravital Imaging of Vascular Transmigration by the Lyme ... - PLOS
2015年12月18日 · Here we describe a novel approach to study transmigration of Lyme disease spirochetes using intravital microscopy of the peripheral knee vasculature in living mice. Our studies have identified an adhesin, P66, and its integrin-binding function as playing important roles in Lyme spirochete transmigration and dissemination.
Nanomechanical mechanisms of Lyme disease spirochete motility ...
2021年3月1日 · Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly Decorin...