Luciferase - Wikipedia
Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. The name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, respectively. [1]
一文学习Luciferase（最全） || Luciferase 报告基因系统方法及原 …
2021年5月31日 · Luciferase报告基因系统是以荧光素（luciferin）为底物来检测萤火虫荧光素酶（ fireflyluciferase）活性的一种报告系统。 荧光素酶可以催化 luciferin 氧化成 oxyluciferin，在 luciferin 氧化的过程中，会发出生物荧光（ bioluminescence）。 然后可以通过荧光测定仪也称化学发光仪（ luminometer）或液闪测定仪测定luciferin氧化过程中释放的生物荧光。 荧光素和荧光素酶这一生物发光体系，可以极其灵敏、高效地检测基因的表达。 是检测转录因子与目的 基 …
Luciferase: A Powerful Bioluminescent Research Tool
2024年7月16日 · Luciferase is a group of enzymes that oxidize a substrate known as luciferin to produce light. 1 For bioluminescence, the firefly luciferase enzyme catalyzes the oxidation of D-luciferin in the presence of oxygen (O 2), adenosine triphosphate (ATP), and …
Luciferase | Definition, Bioluminescence, Enzyme, Reaction,
2 天之前 · Luciferase, enzyme manufactured in the cells of certain organisms to control bioluminescence. The widespread bioluminescence of such living organisms as fireflies and bacteria is based on the oxidation of a luciferin molecule in …
Everything About Luciferin and Luciferase - GoldBio
Luciferase is an enzyme that catalyzes a light-producing biochemical reaction when it is in the presence of oxygen and a naturally occurring substrate called luciferin. Because of the light-emitting phenomenon, the luciferase-luciferin enzyme-substrate combination is the basis behind one of nature’s most common forms of bioluminescence.
1001 lights: luciferins, luciferases, their mechanisms of action …
2016年10月24日 · Bioluminescence (BL) is a spectacular phenomenon involving light emission by live organisms. It is caused by the oxidation of a small organic molecule, luciferin, with molecular oxygen, which is catalysed by the enzyme luciferase. In nature, there are approximately 30 different BL systems, of which …
Looking into luciferin - Nature Chemistry
2023年5月5日 · Though collectively referred to as luciferins and luciferases, in different organisms they correspond to distinct substrates and enzymes that do not necessarily cross-react. Today, we know that...
Luciferin - an overview | ScienceDirect Topics
The enzyme luciferase catalyses the oxidation of luciferin as a substrate in the presence of adenosine triphosphate (ATP) (Scheme 7). Another bioluminescence system makes use of a luciferase from certain marine bacteria.
Leaving the Dark Side? Insights Into the Evolution of Luciferases
2021年6月29日 · Some marine copepods emit a bright blue light using a classical luciferase-luciferin system based on the coelenterazine substrate. It is a case of secreted bioluminescence, and the simple oxidation reaction do not require any additional cofactors. Copepod luciferases are small secreted proteins of around 18-24 kDa.
Luciferin - Wikipedia
Luciferins are a class of small-molecule substrates that react with oxygen in the presence of a luciferase (an enzyme) to release energy in the form of light. It is not known just how many types of luciferins there are, but some of the better-studied compounds are listed below.