Institut Pasteur MLST databases and software
BIGSdb-Lm provides access to genotypic data for Listeria isolates based on Multilocus Sequence Typing (MLST), core genome MLST (cgMLST) and other schemes. The database is now also accessible through RESTful API (see below).
Listeria PasteurMLST database - Institut Pasteur
The Listeria PasteurMLST database contains data for a collection of isolates that represent the total known diversity of Listeria species. For every allelic profile in the profiles/sequence definition database there is at least one corresponding isolate deposited here.
Listeria locus/sequence definitions database - Institut Pasteur
The Listeria PasteurMLST sequence definition database contains allele and profile data representing the total known diversity of Listeria species. Every new ST deposited in this database should have a corresponding record in the isolate database .
Genomic Characterization of Listeria monocytogenes Isolated …
2020年6月25日 · Core genome MLST (cgMLST) analysis classified isolates into seven sublineages (SL) and 25 different cgMLST types (CT). Consistent with the MLST results, most sublineages were SL2 and SL5.
Multilocus Sequence Typing (MLST) of Listeria monocytogenes
2014年1月1日 · Nucleotide sequence-based methods focusing on the single nucleotide polymorphisms (SNPs) of Listeria monocytogenes housekeeping genes facilitate the rapid and interlaboratory comparison on open accessible databases, such as the multilocus sequence typing (MLST) databases that are available.
Assessment of the relationship between the MLST genetic
Listeria monocytogenes can grow under stressful conditions and contaminate various food categories. Progresss in DNA sequencing-based identification methods, such as multi-locus sequence typing (MLST) now allow for more accurate characterization of pathogens.
Analysis of Multilocus Sequence Typing and Virulence …
2016年2月16日 · Multilocus sequence typing (MLST) method is one of the most robust tools for investigating the global epidemiology of microbial populations (Sullivan et al., 2005 ). Based on the sequencing of seven housekeeping genes, it is highly discriminatory and provides unambiguous results that can be comparable directly among laboratories via the internet.
In vitro and in silico parameters for precise cgMLST typing of Listeria …
2022年3月26日 · Whole genome sequencing analyzed by core genome multi-locus sequence typing (cgMLST) is widely used in surveillance of the pathogenic bacteria Listeria monocytogenes. Given the heterogeneity of available bioinformatics tools to define cgMLST alleles, our aim was to identify parameters influencing the precision of cgMLST profiles.
Comparative Analysis of Multilocus Sequence Typing and Pulsed …
One hundred seventy-five Listeria monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) based on loci in actA, betL, hlyA, gyrB, pgm, and recA.
Multilocus Sequence Typing (MLST) and Whole Genome
2020年9月26日 · Nucleotide sequence-based methods focusing on the single-nucleotide polymorphisms (SNPs) of Listeria monocytogenes and L. innocua housekeeping genes (multilocus sequence typing) and in the core genome (core genome MLST) facilitate the rapid and interlaboratory comparison in open accessible databases as provided by Institute Pasteur (https ...