Zooming into lipid droplet biology through the lens of electron ...
2024年5月10日 · The use of electron microscopy (EM) has proved instrumental in studying lipid droplets (LDs), and the adoption of the appropriate EM methodology is crucial to ensuring an adaqua characterization of t...
Imaging Lipid Droplets by Electron Microscopy - ScienceDirect
2013年1月1日 · In this chapter, we discuss methods that have been used and/or are potentially useful to study LDs. The methods include conventional EM to observe the LD core, cryoelectron microscopy to observe the LD surface, freeze-substitution, immunoelectron microscopy (pre-embedding, post-embedding, and cryosectioning methods), and freeze-fracture.
Imaging lipid droplets by electron microscopy - PubMed
The lipid droplet (LD) is different from other cellular organelles in that most of its volume is made of lipid esters and its surface is lined by a phospholipid monolayer. This uniquely lipid-dominant structure poses a problem for electron microscopy (EM) …
Lipid droplets as endogenous intracellular microlenses
2021年12月6日 · Here we show that endogenous lipid droplets in the mature adipose cells can behave as fully biocompatible microlenses to strengthen the ability of microscopic imaging as well as detecting intra-...
Lipid-droplet-accumulating microglia represent a dysfunctional …
2020年1月20日 · Here we report a striking buildup of lipid droplets in microglia with aging in mouse and human brains. These cells, which we call ‘lipid-droplet-accumulating microglia’ (LDAM), are defective...
Zooming into lipid droplet biology through the lens of electron ...
Electron microscopy (EM), in its various flavors, has significantly contributed to our understanding of lipid droplets (LD) as central organelles in cellular metabolism. For example, EM has illuminated that LDs, in contrast to all other cellular organelles, are uniquely enclosed by a …
2024年5月10日 · Electron microscopy (EM), in its various flavors, has significantly contributed to our understanding of lipid droplets (LD) as central organelles in cellular metabolism.
Integrating cellular electron microscopy with multimodal data to ...
2024年2月1日 · In this Review, we detail the latest advancements in volume electron microscopy (vEM) and cryo-electron tomography (cryo-ET), which together can visualize biological complexity across scales from the organization of cells in large tissues to the molecular details inside native cellular environments.
Leading the way in the nervous system: Lipid Droplets as new …
2021年1月1日 · This lipid droplet-laden microglia start hiking inflammation and ROS while having little ability to phagocytose debris, thus contributing to a damaging pro-inflammatory environment in the brain while stimulating ROS-mediated protein aggregation.
Visualization of Lipid Droplets in C. elegans by Light and Electron ...
2013年1月1日 · Methods to visualize these markers using sensitive confocal microscopy systems are detailed. Additional methods for visualizing lipid droplets by transmission electron microscopy and detection of lipid droplet associated proteins by immunoelectron microscopy are described.