CRISPR 101: Homology Directed Repair - Addgene
The conservative methods are characterized by use of a homologous donor (e.g., sister chromatid, plasmid, etc.), and consists of three pathways: classical double-strand break repair (DSBR), synthesis-dependent strand-annealing (SDSA), and break-induced repair (BIR).
Stimulation of CRISPR-mediated homology-directed repair by an ... - Nature
2019年7月30日 · Precise editing of genomic DNA can be achieved upon repair of CRISPR-induced DNA double-stranded breaks (DSBs) by homology-directed repair (HDR). However, the efficiency of this process is...
DNA repair pathway choices in CRISPR-Cas9 mediated genome …
These repair pathways include homologous recombination (HR), classical non-homologous end joining (cNHEJ), microhomology-mediated end joining (MMEJ) and single-strand annealing (SSA). In this review, we briefly highlight the CRISPR-Cas9 system and we then describe the mechanisms of DSB repair.
Efficient high-precision homology-directed repair-dependent …
2023年7月20日 · Finally, DSBs can be repaired by homology-directed repair (HDR) using sister chromatids as templates, referred to as homologous recombination (HR).
Towards a CRISPeR understanding of homologous recombination …
Homologous recombination (HR) is a genome maintenance pathway required for the accurate repair of DNA double-strand breaks (DSBs). HR competes with non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ) for the repair of DSBs, and the choice between these pathways is dictated by the resection of DSB ends [ 1 ].
Increasing Cas9-mediated homology-directed repair efficiency …
2018年5月31日 · The potential of CRISPR-Cas9 to deliver precise genome editing is hindered by the low efficiency of homology-directed repair (HDR), which is required to incorporate a donor DNA template...
Massively parallel CRISPR-assisted homologous recombination …
2024年5月15日 · Coupled with massively parallel DNA design and synthesis, we developed a saturation gene editing method termed CRISPR-Cas9– and homology-directed repair–assisted saturation editing (CHASE) and achieved highly saturated codon swapping of …
Boosting CRISPR-Cas9 efficiency through enhanced homologous recombination
The study by Park et al. demonstrated that concomitant upregulation of RAD51 expression coupled with SCR7-mediated suppression of the NHEJ pathway markedly increases homologous recombination efficiency, thereby optimizing the overall efficacy of genome editing.
Design of Repair Templates for CRISPR-Cas9-Triggered Homologous …
2021年7月31日 · Homologous Recombination (HR) produces specific and precise error-free recombinants. HR requires a repair template carrying homologous DNA sequences both up and downstream to the target site [12].
CRISPR-Cas9-mediated homology-directed repair for precise …
2024年9月27日 · CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating precise site-specific DNA insertions, deletions, and substitutions. These precise edits are made possible through the use of exogenous donor templates that …