Electrophoretic mobility shift assay - Wikipedia
An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common affinity electrophoresis technique used to study protein–DNA or protein – RNA interactions.
Gel Shift Assays (EMSA) | Thermo Fisher Scientific - US
Because the rate of DNA migration is shifted or retarded when bound to protein, the assay is also referred to as a gel shift or gel retardation assay. The ability to resolve protein–DNA complexes depends largely upon the stability of the complex during each step of the procedure.
Electrophoretic Mobility Shift Assay (EMSA) for Detecting ...
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the characterization of interacting systems.
The Gel Retardation Assay - SpringerLink
The gel retardation or electrophoretic mobility shift assay (EMS A) is a sensitive technique for studying protein–DNA interactions. Originally devised by Fried and Crothers (1) to study the kinetics of protein‐DNA interactions, the method relies on the...
Electrophoretic mobility shift assay (EMSA) for detecting ...
2007年7月26日 · The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids.
Gel Mobility Shift Assays to Detect Protein–RNA Interactions
Gel mobility shift (gel shift) assays, also referred to as electrophoretic mobility shift assays (EMSA), allow rapid detection and quantification of protein–RNA interactions (1 – 5). In vitro generated RNA is typically end-labeled at the 5′ end with [γ- …
Electrophoretic Mobility Shift Assay - an overview ...
Electrophoretic mobility shift assay, also known as gel shift or gel retardation assay, was originally developed for the evaluation of protein–DNA binding affinity (Fried and Crothers, 1981). In the context of RNAi delivery, it is a common method to illustrate the formation of oligonucleotide–carrier complexes.