Circulating tumor cells in breast cancer: clinical validity ... - Nature
6 天之前 · Circulating tumor cells (CTCs) have been extensively studied in breast cancer (BC), with large studies establishing CTCs as a robust prognostic biomarker in early and metastatic breast cancer (MBC).
Detection of circulating tumor cells: Advances and critical concerns
Isolation of circulating tumor cells by immunomagnetic enrichment and fluorescence-activated cell sorting (IE/FACS) for molecular profiling. ... Babu KG, Rangarajan A. A novel ex vivo system using 3d polymer scaffold to culture circulating tumor cells from breast cancer patients exhibits dynamic E-M phenotypes. J Clin Med. 2019;8:1473. doi: 10. ...
Identification of Atypical Circulating Tumor Cells with Prognostic ...
In this study we have isolated and analyzed atypical cells found in the blood of metastatic breast cancer patients using a micro-filtration technic. This technic, being very easy to implement, was also extremely useful for studying circulating ...
Breast cancer circulating tumor cells with mesenchymal …
To examine which methods are the most appropriate for recovery of mesenchymal BC CTCs, we performed literature review to search for studies which have performed spike-in experiments with BC cell lines with a variety of CTCs isolation methods and retrieved the information on the mean RRs (± SD) and number of test repeats (n).EMT score of BC cell lines was obtained from literature [] and used ...
Circulating tumor cell isolation for cancer diagnosis and prognosis
Circulating tumor cells (CTCs) are tumor cells that shed from the primary tumor and intravasate into the peripheral blood circulation system responsible for metastasis. Sensitive detection of CTCs from clinical samples can serve as an effective tool in cancer diagnosis and prognosis through liquid biopsy.
Microfluidic isolation of breast cancer circulating tumor cells …
2023年8月2日 · In this study, we use an inertial microfluidic device reported earlier to capture cancer cells from mouse blood, followed by enumeration of the isolated cells using cytocentrifugation, and highlight the sources of cell loss in the isolation protocol.
Rapid Isolation and Detection of Breast Cancer Circulating Tumor Cells ...
2024年4月3日 · Circulating tumor cells (CTCs) have garnered special attention as promising cancer biomarkers. Phenotypic changes of CTCs reveal invaluable information for oncologists in disease prognosis and adjusting their treatment options.
Circulating tumour cell clusters: isolation, biological significance ...
2024年9月10日 · Circulating tumour cells (CTCs) and CTC clusters are considered metastatic precursors due to their ability to seed distant metastasis. However, navigating the bloodstream presents a significant challenge for CTCs, as they must endure fluid shear forces and resist detachment-induced anoikis.
Circulating tumor cells clusters and their role in Breast cancer ...
2024年4月1日 · CTCs can travel alone or in groups called CTC clusters. Studies have shown that CTC clusters have more potential for metastasis and a poorer prognosis than individual CTCs in breast cancer patients. However, our understanding of CTC clusters' formation, structure, function, and detection is still limited.
Microfluidic isolation of breast cancer circulating tumor cells …
2023年8月2日 · Here, we demonstrate and evaluate a complete workflow of a sized-based inertial microfluidic device to isolate CTCs from blood using exclusively mouse blood and mouse cancer cell lines. We then incorporate the cytospin, a commonly used method for enumeration of small number of cells in a glass slide to quantify the total cell yield of our workflow.