Microbial production of recombinant chymosin - microbewiki
2013年4月29日 · Chymosin, commonly known as rennin, is the main milk-coagulating enzyme that consists of a single polypeptide chain of 323 amino acids with intramolecular disulfide linkages. Preparations of calf rennet contain two forms of chymosin, A and B.
Production of Bioactive Recombinant Bovine Chymosin in …
Here, we present an alternative method to producing bovine chymosin from transgenic tobacco plants. The CYM gene, which encodes a preprochymosin from bovine, was introduced into the tobacco nuclear genome under control of the viral 35S cauliflower mosaic promoter.
Production of Bioactive Recombinant Bovine Chymosin in …
2016年4月28日 · Chymosin (also known as rennin) plays an essential role in the coagulation of milk in the cheese industry. Chymosin is traditionally extracted from the rumen of calves and is of high cost. Here, we present an alternative method to …
Production of Bioactive Recombinant Bovine Chymosin in …
Here, we present an alternative method to producing bovine chymosin from transgenic tobacco plants. The CYM gene, which encodes a preprochymosin from bovine, was introduced into the tobacco nuclear genome under control of the viral 35S cauliflower mosaic promoter.
Tobacco Plant: A Novel and Promising Heterologous Bioreactor …
2024年1月20日 · Production of recombinant bovine chymosin in plants can be an easy-to-scale-up, safe, and inexpensive platform. Chymosin, also known as renin, is an aspartic endopeptidase that is naturally obtained from the calf's stomach for use in cheese production [1].
Recombinant Lamb Chymosin as an Alternative Coagulating …
2001年5月1日 · The aim of our study was to introduce recombinant lamb chymosin (RLC) as a new, alternative milk coagulating enzyme and evaluate its technological characteristics in com- parison with commercial preparations of recombinant calf chymosin, calf …
Short communication: A comparative analysis of recombinant chymosins ...
2012年2月1日 · Although recombinant bovine chymosin is the most frequently used chymosin in the industry, new sources of recombinant chymosin, such as goat, camel, or buffalo, are now available. The present work represents a comparative study of 4 different recombinant chymosins (goat and buffalo chymosins expressed in Pichia pastoris , and bovine and camel ...
Basic Biochemical Properties of Recombinant Chymosins …
2020年7月28日 · In 2006, the recombinant dromedary (Camelus dromedarius) chimosin (rCn) was discovered and then introduced in the cheesemaking industry [4]. This enzyme outperforms bovine rCn in a number of technological qualities, which makes its use more economically advantageous in a large group of cheeses.
Characterization of recombinant camel chymosin reveals superior ...
2006年4月7日 · Bovine chymosin is the preferred enzyme, combining a strong clotting activity with a low general proteolytic activity. In the present study, we report expression and enzymatic properties of recombinant camel chymosin expressed in Aspergillus niger. Camel chymosin was shown to have different characteristics than bovine chymosin.
We report here the structure‘ of chymosin C formed from recombinant-derived chymosin A, showing that it arisesby chymosin-catalyzed excision of a portion of the chymosin A peptide chain in the region of its single-residue difference from chymosin B. Studies with syn- thetic peptides suggest a likely order of cleavage for the t...