In chemistry, the term chromogen refers to a colourless (or faintly coloured) chemical compound that can be converted by chemical reaction into a compound which can be described as "coloured" (a chromophore).
chromogen, such as Fast Red to produce an insoluble red precipitate at the site of the alkaline phosphatase enzyme. In situations where a brown chromogen is not desirable, other colored chromogens can be used. In particular melanin presents an additional challenge in IHC because this brown pigment provides no visual contrast with the brown DAB
In contrast, a chromogen is a colorless chemical compound that is only converted to a colored compound through a chemical reaction. In IHC, this reaction occurs by the action of enzymes. In the IHC protocol, enzymes are directly or indirectly attached to the antibody-
2021年11月22日 · A chromogen is a colored compound containing a chromophore—responsible for the observed color . A chromophore is a group of atoms within a larger molecule that are principally responsible for the absorption of certain wavelengths of visible light, while transmitting wavelengths that are not absorbed [ 5 ].
A chromogen is a substance that can be converted into a colored compound, often used in immunoassays to indicate the presence of specific antigens or antibodies. In laboratory analysis, chromogens are key components for visualizing results in assays like EIAs and ELISAs.