High-content CRISPR screening | Nature Reviews Methods Primers
2022年2月10日 · We outline good practices for analysing and interpreting data from CRISPR screens, and review groundbreaking applications of CRISPR screening across a broad range …
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening
2014年12月10日 · Here we describe a protocol for genome-scale knockout and transcriptional activation screening using the CRISPR-Cas9 system. Custom- or ready-made guide RNA …
Introduction to CRISPR screening | IDT - Integrated DNA …
2019年11月6日 · CRISPR screening can identify genes that influence many physiological effects, including drug resistance and drug sensitivity. This article provides an overview of how …
CRISPR–Cas9 screens reveal regulators of ageing in neural
2024年10月2日 · Here we develop in vitro and in vivo high-throughput CRISPR–Cas9 screening platforms to systematically uncover gene knockouts that boost NSC activation in old mice.
Genome-wide CRISPR-Cas9 knockout screens - Wikipedia
Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide scale and studying …
CRISPR-Cas and CRISPR-based screening system for precise …
2024年5月30日 · In this review, we summarized the genetic modification by CRISPR/Cas and CRISPR screening system, discussed key components for successful CRISPR screening, …
Research Techniques Made Simple: CRISPR Genetic Screens
In brief, the bacterial Cas enzyme (usually Cas9) is guided to a genomic DNA target by a single guide RNA (sgRNA), an approximately 20-nucleotide sequence that specifies the genomic …
FACS-based genome-wide CRISPR screens define key ... - Cell Press
2023年8月3日 · Besides genetic screens, fluorescence-activated cell sorting (FACS)-based CRISPR-Cas9 screens using reporter cell lines can be used to study specific signaling …
CRISPR/Cas9 screening: unraveling cancer immunotherapy’s …
2024年5月18日 · Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) screening, a potent tool for unbiased functional genomic …
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells …
2013年12月12日 · We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both …