CRISPR Plasmids and Resources - Addgene
Mutated Cas nickase enzymes generate single-strand DNA breaks. dCas9 fused to an activator peptide activates gene expression. dCas9 alone, or fused to a repressor peptide, inhibits transcription. dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression. CRISPR libraries are a powerful tool for genome-wide screening.
CRISPR Guide - Addgene
S. pyogenes Cas9 (SpCas9) is the most commonly used CRISPR endonuclease for genome engineering, but there are other Cas enzymes available. These enzymes have unique properties and can target different types of nucleic acids, including dsDNA (Cas9, Cas12), ssRNA (Cas13), or ssDNA (Cas14).
Cas9质粒-CRISPR载体-赛默飞 | Thermo Fisher Scientific - CN
GeneArt CRISPR核酸酶载体试剂盒整合了Cas9核酸酶试剂盒和靶向向导RNA (gRNA)克隆试剂盒，允许共表达Cas9 和gRNA，在编辑单个靶基因时使用同一个质粒。
CRISPR/Cas9 Plasmids for sgRNA Delivery & Gene Editing
GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol or ID on our gRNA Database. Enhanced CRISPR/SpCas9 Plasmids New!
Cas9 Plasmids | Thermo Fisher Scientific - US
Learn about designing all-in-one CRISPR plasmids for gene editing experiments. GeneArt Nuclease Vector Kits allow for combined expression of Cas9 and gRNA for editing a target gene using a single plasmid.
Genome engineering using the CRISPR-Cas9 system - Nature
2013年10月24日 · The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering...
CRISPR/Cas9 therapeutics: progress and prospects
2023年1月16日 · Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases by...
Successful delivery of large-size CRISPR/Cas9 vectors in hard-to ...
2020年6月19日 · With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in...
CRISPR/Cas9 systems: Delivery technologies and ... - ScienceDirect
2023年11月1日 · In this review, we present a comprehensive outline of delivery strategies and discuss their biomedical applications in the CRISPR/Cas9 system. We also provide an in-depth analysis of physical, viral vector, and non-viral vector delivery strategies, including plasmid-, mRNA- and protein-based approach.
CRISPR Plasmids - SnapGene
CRISPR plasmids for genome editing and gene regulation from Addgene, transOMIC, and others