Chromatography affinity resin with photosynthetically-sourced protein …
2024年4月15日 · In search of valuable and useful protein candidates for photosynthesis-driven mass-production, we successfully engineered tobacco chloroplasts to express a recombinant variant of SpA, producing a...
Chromatography affinity resin with photosynthetically-sourced protein …
2024年4月15日 · In search of valuable and useful protein candidates for photosynthesis-driven mass-production, we successfully engineered tobacco chloroplasts to express a recombinant variant of SpA, producing a transplastomic line, a “green plant bioreactor”, with yields reaching ~ 250 mg of the recombinant protein per 1 kg fresh leaf biomass.
Protein purification with light via a genetically encoded ... - Nature
3 天之前 · Therefore, the affinity-purified protein normally has to undergo an additional purification step, often size exclusion chromatography (SEC) or ion exchange chromatography (IEC), but at least a ...
Aptamer-Based Affinity Chromatography for Protein Extraction …
2019年9月5日 · In this review we will first present a short historical recall of the ligand evolution in affinity chromatography and then focus the attention of the reader on effective ways of selecting single-chain oligonucleotide affinity ligand molecules in view of protein purification.
A Chimeric Affinity Tag for Efficient Expression and …
2016年2月15日 · In this study, we designed the Cysta-tag, a new purification tag for immobilized metal affinity chromatography (IMAC) of plant-made proteins based on the protein-stabilizing fusion partner SlCYS8. We show that the Cysta-tag may be used to readily purify proteins under native conditions, and then be removed enzymatically to isolate the protein ...
Efficient development of nanobody-based affinity chromatography …
To acquire purified protein for AAV8 affinity chromatography resin preparation, the supernatant containing Nb9 (tagged with an N-terminal His6) from a 7L fermenter was sequentially processed through IMAC resin followed by size-exclusion chromatography (SEC). The purification chromatograms for the two steps were presented in Fig. 6 A and B.
Heterologous expression, purification, and biochemical …
5 天之前 · After expression, the expressed protein was purified by Ni-NTA affinity chromatography. To characterize protease 3075 structural and biochemical features, the kinetic parameters, pH profiling, thermal stability, thermodynamics parameters, molecular docking, and molecular dynamics simulation (MDS) were applied to evaluate the potential ...
Frontiers | A Chimeric Affinity Tag for Efficient Expression and ...
2016年2月14日 · In this study, we designed the Cysta-tag, a new purification tag for immobilized metal affinity chromatography (IMAC) of plant-made proteins based on the protein-stabilizing fusion partner SlCYS8.
Exploring affinity chromatography in proteomics: A …
2024年6月1日 · Affinity chromatography separates molecules based on their specific interactions. In proteomics, affinity separation aids protein and peptide depletion or enrichment. Three main areas of proteomic affinity chromatography are explored in detail. Recent developments in affinity ligands and approaches in proteomics are discussed.
A Comparative Study of Lectin Affinity Based Plant N …
2014年2月1日 · Lectin affinity chromatography (LAC) can provide a valuable front-end enrichment strategy for the study of N -glycoproteins and has been used to characterize a broad range eukaryotic N -glycoproteomes. Moreover, studies with mammalian systems have suggested that the use of multiple lectins with different affinities can be particularly effective.