Adenylate kinase - Wikipedia
Adenylate kinase (EC 2.7.4.3) (also known as ADK or myokinase) is a phosphotransferase enzyme that catalyzes the interconversion of the various adenosine phosphates (ATP, ADP, and AMP).
Adenylate Kinase - an overview | ScienceDirect Topics
Adenylate kinase (AK) is a constitutively expressed enzyme which comprises about 0.1% of cell protein. It catalyses the equilibrium reaction: where ATP, ADP and AMP are the adenosine tri-, di- and monophosphates respectively.
The energy landscape of adenylate kinase during catalysis
Here we have performed a comprehensive investigation of the adenylate kinase (Adk) energy landscape and have quantified multiple kinetic states along the reaction pathway at atomic resolution. Adk is a ubiquitous and essential phosphoryl-transfer enzyme found in all cells.
Adenylate Kinase - an overview | ScienceDirect Topics
Adenylate Kinase is a housekeeping enzyme involved in energy metabolism through the catalysis of the nucleotide exchange reaction 2ADP ↔ ATP + AMP. It plays a crucial role in various cellular processes such as hormone secretion, endocytosis, cellular motility, nuclear transport, and energetic metabolic signaling pathways.
Insights into Enzymatic Catalysis from Binding and Hydrolysis of ...
2023年7月7日 · Here, we explore how adenylate kinase (AdK) from Escherichia coli interacts with diadenosine tetraphosphate (AP4A), a putative alarmone associated with transcriptional regulation, stress, and DNA damage response.
Magnesium induced structural reorganization in the active site of ...
2024年8月9日 · To determine the K m and the reaction velocity at saturating substrate conditions k cat, the Michaelis-Menten equation was fitted to the normalized reaction velocities (V/[E] tot) in response to increasing substrate concentrations ([S]). Errors were estimated by performing the experiment in triplicate
Probing the transition state in enzyme catalysis by high ... - Nature
2019年6月24日 · Here, we develop a synergistic computational/experimental approach that exploits the kinetic dependence of protein motion with hydrostatic pressure to characterize the structure of the TSE during...
How is AMP "recharged" to become ATP in a cell?
2018年11月20日 · AMP is first converted to ADP in the reaction. AMP + ATP $\leftrightarrow$ 2 ADP. catalyzed by adenylate kinase. So one phophate group is transfered from ATP to AMP, resulting in two ADP molecules. The ADP formed is then used to synthesize ATP as usual, by the mitochondrial ATP synthase or by the glycolysis enzymes.
The energy landscape of adenylate kinase during catalysis
2015年1月12日 · Structural, computation and kinetics approaches reveal the energy landscape of catalysis by adenylate kinase and show that the cofactor Mg2+ activates two distinct molecular events in the...
Regulation of Adenine Nucleotide Metabolism by Adenylate Kinase …
2023年3月14日 · Adenylate kinase (AK) regulates adenine nucleotide metabolism and catalyzes the ATP + AMP ⇌ 2ADP reaction in a wide range of organisms and bacteria. AKs regulate adenine nucleotide ratios in different intracellular compartments and maintain the homeostasis of the intracellular nucleotide metabolism necessary for growth, differentiation, and ...
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