The ABI 3900 High-Throughput DNA Synthesizer is an economical, versatile platform that synthesizes DNA oligos with rapid cycle times, low reagent consumption, and incomparable reliability.
RNA synthesis chemistry has been recently refined to use DMT protected cyanoethyl RNA phosphoramidites on your Applied Biosystems DNA synthesizer. Oligoribonucle-otides of high purity and yield have been obtained on the models 380A, 380B, 381A and 391 EP AB oligonucleotide synthesizers.
A 21-mer oligonucleotide was synthesized on a Model 380B DNA Synthesizer primer equipped with both 40-nanomole polystyrene and 0.2-μmole CPG columns. Four milli-grams of phosphoramidite were delivered each cycle to test the supports stringently, using the fast cycle. Figure 1. CEs of crude 21-mers.
date of shipment to the Customer ("the Warranty Period"), the Applied Biosystems Model 380B purchased by the customer (the "Instrument") will be free from defects in material and workmanship, and will perform in accordance with the performance specifications contained in the applicable Applied Biosystems product literature (the "Specifications").
Included on both disks are fast cycles for standard small-scale (0.2-pmole), 1.0-gmole, and 10.0-gmole cyanoethyl DNA synthesis and for RNA synthesis. Also included are cycles for hydrogen-phosphonate DNA synthesis.
Which DNA synthesizer should I buy? - ResearchGate
We usually use a synthesis scale of around 200nM or less, and it would be enough for us to have a synthesis capacity of 100 oligos/month including regular oligos (desalted) and Taqman Probes.
Phosphalink allows the convenient addition of a phosphate group at the 5' and/or 3' ends of oligonucleotides with high coupling yield.
Oligoribonucleotide - an overview | ScienceDirect Topics
The DNA oligonucleotides are synthesized on a PE-ABI 380B automated DNA synthesizer and standard phosphoramidite chemistry. The DNA oligonucleotides were purified by precipitation 2 times out of 0.5 M NaCl with 2.5 volumes of ethanol.
8 questions with answers in DNA SYNTHESIZER | Science topic
2015年5月1日 · Explore the latest questions and answers in DNA Synthesizer, and find DNA Synthesizer experts.
Oligo-2′-Fluoro-2′-Deoxynucleotide N3′→P5′ Phosphoramidates: Synthesis …
1996年8月1日 · Uniformly modified oligodeoxyribonucleotide N3′→P5′ phosphoramidates containing 2′-fluoro-2′-deoxy-pyrimidine nucleosides were synthesized using an efficient interphase amidite transfer reaction.