微生物鉴定——酵母菌的培养及鉴定流程 - 知乎
2、26s rrna基因的pcr扩增与序列测定. 与细菌不同的是，酵母菌的鉴定一般通过26s rrna（600 bp左右）就可以一步将其鉴定到种水平。26s rrna基因序列扩增引物：正向 nl⁃1：（5’⁃ gcatatcaataagcggaaaag ⁃3’ ）和反向引 物 nl⁃4：（5’⁃ ggtccgtgtttcaagacgg ⁃ 3’ ）。
为什么酵母菌使用26srRNA进行分子生物学鉴定 - Baidu
26S rRNA是真菌细胞的一个高度保守的结构RNA，在不同物种间具有较大的序列不同性，可以用于真菌的分类和鉴定。 通过对26S rRNA进行测序和序列比对，可以准确地鉴定出真菌菌株的种属和亚种，能够帮助生物学家更准确地了解真菌物种的多样性和分布情况，开展真菌资源的保护和利用。 此外，通过对真菌群落中的26S rRNA进行高通量测序，可以深入了解真菌菌群的组成和多样性，从而为研究真菌的生态、功能以及相互作用等方面提供更为全面准确的信息。 因此，26S …
The primary and secondary structure of yeast 26S rRNA.
2001年1月1日 · We present the sequence of the 26S rRNA of the yeast Saccharomyces carlsbergensis as inferred from the gene sequence. The molecule is 3393 nucleotides long and consists of 48% G+C; 30 of the 43 methyl groups can be located in the sequence.
解脂耶氏酵母26s rDNA：理想的迭代基因整合位点 - 知乎
2023年12月14日 · ARO1 的整合可能存在三种情况，即： (1) 随机整合在26s rDNA位点； (2) 整合并取代先前的柚皮素的代谢途径； (3) 整合在内源的 ARO1 位点。 但是经研究发现， ARO1 的整合效率达到95.8%，且柚皮素的代谢途径以及内源的 ARO1 位点均没有被破坏，说明 ARO1 被整合到26s rDNA的其他位点，26s rDNA可以作为靶点进行多次迭代基因整合。 之后，为了提高柚皮素产量，乙酰辅酶A合酶 ACS 以及羧化酶 ACC1 被多拷贝迭代整合，产量提高62.6%。 最后， …
Yeast identification by the 26S rRNA D1/D2 domains sequencing.
Yeast identification by the 26S rRNA D1/D2 domains sequencing. The influence of fruit varieties on yeast ecology during spontaneous plum mash fermentation was investigated. Yeast colonies were...
In this investigation 17S and 26S rRNA are shown to have certain structural differences as illustrated by the distinct electrophoretic patterns of the ribonuclease T 1 digests of the two RNAs and...
The 26S rRNA binding ribosomal protein equivalent to bacterial …
1990年8月8日 · Transformant phages expressing L15, a yeast ribosomal protein which binds to 26S rRNA and interacts with the acidic ribosomal proteins, were isolated by screening a yeast cDNA expression library in lambda gt11 with specific monoclonal antibodies.
The primary and secondary structure of yeast 26S rRNA
1981年12月21日 · We present the sequence of the 26S rRNA of the yeast Saccharomyces carlsbergensis as inferred from the gene sequence. The molecule is 3393 nucleotides long and consists of 48% G+C; 30 of the 43 methyl groups can be located in the sequence.
Cryo-EM structure of the RNA-rich plant mitochondrial ribosome
2020年4月6日 · The 26S, 18S and 5S rRNAs are 3,169, 1,935 and 118 nucleotides in length, respectively, therefore making the plant mitochondria SSU and LSU rRNAs 20% and 9% larger than their prokaryote...
Concerted and birth-and-death evolution of 26S ribosomal DNA …
2020年9月17日 · Ribosomal RNA (rRNA), accounting for ~80 % of cellular RNA and 20 % of total cell dry weight, is closely related to the growth and development of the organism (Gourse et al., 1996). In eukaryotes there are four types of rRNA molecule (18S, 5.8S, 26S and 5S), which are encoded by nuclear ribosomal DNA (rDNA).
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