Genetic transduction by phages and chromosomal islands: The …
2019年8月8日 · Generalized transduction, discovered in Salmonella phage P22, was the first mechanism of phage-mediated gene transfer to be identified . It is the process by which phages can package any bacterial DNA (chromosomal or plasmid) and transfer it to another bacterium.
Bacterial Transduction: Definition, Principle, Steps, Examples
2023年7月11日 · Transduction is the transfer of bacterial DNA from a donor to a recipient bacterium via a virus particle. The virus particle that infects bacteria is called a bacteriophage or phage, and the phages used for the transfer of DNA are called transfusing phages. Not all phages are transducing phages.
7.11C: Bacterial Transduction - Biology LibreTexts
2024年11月23日 · Transduction is a method for transferring genetic material. The packaging of bacteriophage DNA has low fidelity and small pieces of bacterial DNA, together with the bacteriophage genome, may become packaged into the bacteriophage genome.
Bacteriophages benefit from generalized transduction - PMC
Here we show that generalized transduction, one type of bacterial DNA transfer by phages, can create conditions where not only the recipient host but also the transducing phage benefit.
Bacteriophage-Mediated Horizontal Gene Transfer: Transduction …
2021年1月31日 · Transduction occurs when a bacteriophage mistakenly packages bacterial DNA into a phage particle (transducing particle), either in place of, or in addition to its own genome. This chapter considers this role of phages in the horizontal transfer of DNA between bacteria.
Phage Transduction - PubMed
Bacteriophages mediate horizontal gene transfer through a mechanism known as transduction. Phage transduction carried out in the laboratory involves a bacterial donor and a recipient, both of which are susceptible to infection by the phage of interest.
ProtocolsP1Transduction - Barrick Lab
If using the lysate for the first time or troubleshooting a transduction, try varying the amount of lysate that is added to the cells - ex: up to 200µl. To date, 1-5µl has proven sufficient for P1 Transductions using the deconvoluter donor strains. Allow the phage to …
Lateral transduction is inherent to the life cycle of the ... - Nature
2021年11月8日 · Here, the authors show that prophage excision is delayed in Salmonella phage P22, thus allowing the packaging and transfer of large fragments of host DNA via lateral transduction.
Bacteriophage Mediates Efficient Gene Transfer in Combination …
To allow phage entry into mammalian cells to achieve gene delivery, we evaluated the bacteriophage vector system in combination with chemical modifications of the phage capsid using transduction-enhancing agents.
Phage Transduction - SpringerLink
2016年8月10日 · Bacteriophages mediate horizontal gene transfer through a mechanism known as transduction. Phage transduction carried out in the laboratory involves a bacterial donor and a recipient, both of which are susceptible to infection by the phage of interest.
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