Actin bundle architecture and mechanics regulate myosin II …
2021年5月18日 · Here, we use fluorescence microscopy and simulations to investigate how actin bundle architectures with varying polarity, spacing, and rigidity impact myosin II dynamics and force generation.
A binding protein regulates myosin-7a dimerization and actin bundle ...
2021年1月25日 · Here, we identify a binding protein for Drosophila myosin-7a termed M7BP, and describe how M7BP assembles myosin-7a into a motile complex that enables cargo translocation and actin...
Flagella-like beating of actin bundles driven by self-organized myosin …
2022年8月8日 · Here we show that in the presence of myosin motors, polymerizing actin filaments self-assemble into polar bundles that exhibit wave-like beating. Importantly, filament beating is associated...
Self-organized spatial targeting of contractile actomyosin rings for ...
2024年11月29日 · Incorporating this spatial positioning tool, able to induce active transport of membrane-attached diffusible molecules, yields self-organized equatorial assembly of actomyosin rings in vesicles....
A binding protein regulates myosin-7a dimerization and actin bundle ...
Here, we identify a binding protein for Drosophila myosin-7a termed M7BP, and describe how M7BP assembles myosin-7a into a motile complex that enables cargo translocation and actin cytoskeletal remodeling. M7BP binds to the autoinhibitory tail of myosin-7a, extending the molecule and activating its ATPase activity.
By using simulation and in vitro reconstitution, we demonstrate that the actin bundle architecture (interfilament spacing and filament polarity) and cross-linker mechanics impact the duration, direction, and magnitude of myosin-based force generation.
Actin bundle architecture and mechanics regulate myosin II …
2021年5月18日 · Here, we use fluorescence microscopy and simulations to investigate how actin bundle architectures with varying polarity, spacing, and rigidity impact myosin II dynamics and force generation. Microscopy reveals that mixed-polarity bundles formed by rigid cross-linkers support slow, bidirectional myosin II filament motion, punctuated by periods ...
Myosin-driven actin-microtubule networks exhibit self-organized …
2021年2月5日 · Here, we create a network of co-entangled actin and microtubules driven by myosin II. We combine dynamic differential microscopy, particle image velocimetry, and particle tracking to show that both actin and microtubules undergo ballistic contraction with unexpectedly indistinguishable characteristics.
Myosin III-mediated cross-linking and stimulation of actin bundling ...
2016年1月19日 · As such, our study reveals a previously unrecognized direct myosin binding-induced actin bundling activity regulation mechanism of an actin filament cross-linker protein. It also demonstrates an intimate synergistic action mechanism between two classes of actin binding proteins, namely actin filament-based myosin motors and actin filament cross ...
Parallel actin bundles and their multiple actin-bundling proteins
2000年2月1日 · In the past 18 months, new findings have solidified the concept that parallel actin bundles are assembled in cells through the sequential action of multiple actin-bundling proteins and have begun to shed light on the roles played by the individual actin-bundling proteins.