Using this technique, the relative mRNA levels of genes showing cell type–specific expression can be accurately determined even in tissue samples containing a heterogeneous blend of cells of ...

Rosalind Franklin, James Watson and Francis Crick discovered the structure of DNA—that molecular blueprint for life—over 70 ...

Methods Total mRNA isolated from ∼100mg tissue samples of normal (n=9) and pathologic (n=12) tendon was assayed by relative quantitative RT-PCR for MMP (-1, -2, -3, -9, and -13), ADAMTS (-1, -4, -5), ...

When a gene is to be expressed, the base sequence of DNA is copied or transcribed into mRNA (messenger RNA). This process takes place in the nucleus and occurs in a series of stages. The two ...