1| In a sterile 0.5-ml microcentrifuge tube or amplification tube (on ice), mix the following reagents for the first amplification reaction: 2| Place the tubes in a thermocycler and amplify the ...

1| Add 0.001–100 ng of poly(A) + mRNA or 0.01–1 μg of total RNA into each of three microcentrifuge tubes. Adjust the volumes to 9 μl with water. Denature the RNA by heating at 75 °C for 5 ...

The BD Accuri C6 Cytometer with automated, multisampler is an easy to use cell analyzer that supports the use of any brand of tube, including microcentrifuge tubes, and 12 x 75 mm tubes made from ...