For example, Millipore supplies the QCM Quantitative Cell Migration assay that uses an 8-μM pore size membrane, optimal for epithelial and fibroblast cell migration assays. The company Neuro ...
One of the more commonly used methods is the Boyden chamber (also called trans-well migration assay), whereby cells placed in inserts are allowed to invade and migrate through an artificial ...
Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...
Endothelial cell migration assay was performed in a modified Boyden chamber. Nuclear factor-κB (NF-κB) was studied by stably transfecting HMEC-1 cells with a cis-reporter plasmid containing ...
Our studies suggest that components of the extracellular matrix (collagens, proteoglycans, coagulation proteins) may directly influence the growth, migration and functional ... agents using in vitro ...
Angiogenesis is a fundamental process underlying the pathology of many diseases. It can be modeled in vitro using tube formation assays, in which endothelial cells are induced to form tube-like ...