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![A Python script to design primers for overlap extension PCR to ligate two DNA fragments [PeerJ]](/images/search?view=detailV2&id=A0D598F491CC3703FB7F58FCE0B6ED0801290B74&thid=OIP.42BqynZO0oMCRRZ_KgeJfQHaFY&mediaurl=https%3a%2f%2fdfzljdn9uc3pi.cloudfront.net%2f2022%2f14283%2f1%2ffig-1-2x.jpg&exph=872&expw=1200&q=primer+design+for+sequencing&simid=607999857730327028&ck=6BCA84B058478BF4490DF7CEBF371510&itb=0&FORM=IVCLIG)
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